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KMID : 0921620120420030224
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Journal of Bacteriology and Virology
2012 Volume.42 No. 3 p.224 ~ p.231
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Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice
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Chu Jia-Qi
Kim Su-Mi
Lee Kwang-Nyeong
Oem Jae-Ku
Ko Young-Joon
Lee Hyang-Sim
Kim Yong-Joo
Park Jee-Yong
Kim Kwang-Jae
Parida Satya
Oh Yoon-I
Paton David J.
Joo Yi-Seok
Kim Byoung-Han
Park Jong-Hyeon
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Abstract
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Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon-α (Ad-HIFNα) or pig interferon-β fused with interleukin-18 (Ad-PIFNβ-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFNα, Ad-PIFNβ-IL18, and cocktail of Ad-HIFNα and Ad-PIFNβ-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.
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KEYWORD
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FMD, Interferon, Protection, Adenovirus
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